[Remote] Scientist II - Sr. Scientist, CRISPR Specificity & Genomic Safety

Note: The job is a remote job and is open to candidates in USA. Profluent is a frontier AI lab for biology developing foundation models for biological molecules and therapeutic applications. The company is seeking a Scientist II–Senior Scientist to build and lead CRISPR base-editor specificity and genomic safety workflows, generate NGS-based datasets, and guide editor design and therapeutic program development. The role collaborates across protein engineering, cell biology, bioinformatics, automation, and program teams.

Responsibilities

• Build and lead Profluent’s strategy for base editor specificity assessment, with an emphasis on base-editor-relevant off-target discovery and validation workflows such as CHANGE-Seq-BE and related biochemical, cell-based, and targeted sequencing approaches
• Develop, optimize, benchmark, and apply orthogonal methods to evaluate editing specificity, including workflows for base editors, nucleases, and broader genomic integrity assessment across relevant experimental systems
• Design and execute studies to identify, prioritize, and validate potential off-target editing events, integrating assay development, NGS library preparation, sequencing QC, and data interpretation in partnership with computational biology and program teams
• Generate high-quality, decision-enabling datasets that inform editor engineering, guide/editor selection, delivery conditions, candidate nomination, and program development strategy
• Collaborate cross-functionally with protein engineering, cell biology, gene editing, bioinformatics, automation, and program teams to integrate specificity workflows into Profluent’s broader R&D infrastructure
• Establish robust experimental processes, documentation, and analysis frameworks to support reproducibility, scalability, assay qualification, and potential regulatory-facing genomic safety packages
• Present results and clearly communicate experimental strategy, timelines, risks, milestones, and program implications to diverse scientific and company stakeholders
• Provide mentorship and guidance to junior team members and peers, helping align goals, troubleshoot experiments, and maintain high standards for execution and interpretation
• Stay current with emerging technologies in CRISPR specificity, base editing, off-target discovery, targeted validation, genomic integrity assessment, and therapeutic genome editing

Skills

• PhD (or equivalent) in molecular biology, genomics, genome editing, biochemistry, bioengineering, genetics, chemical biology, or a related field with 2-4 years of relevant industry experience in genome editing specificity and genomic safety assessment
• Deep expertise and hands-on experience with CRISPR-based genome editing systems, particularly base editors, and a strong understanding of modality-specific specificity risks such as DNA off-target editing, RNA off-target editing, bystander editing, indels, and unintended editing outcomes
• Hands-on experience developing, optimizing, or applying NGS-based workflows for genome editing analysis, such as CHANGE-seq, CHANGE-seq-BE, GUIDE-seq, SITE-seq, CIRCLE-seq, Digenome-seq, DISCOVER-seq, INDUCE-seq, targeted amplicon sequencing, rhAmpSeq/AmpSeq-like workflows, hybrid capture, RNA-seq, WGS, or related approaches
• Strong experimental background in molecular biology, mammalian cell culture, genome editing reagent delivery, NGS library preparation, assay troubleshooting, and quantitative analysis of editing outcomes
• Experience interpreting complex sequencing datasets and collaborating with bioinformatics or computational biology teams to analyze off-target discovery, targeted validation, variant calling, RNA editing, indel profiles, or genomic integrity data
• Ability to design rigorous experiments with appropriate controls, replicates, sensitivity considerations, and clear decision criteria
• Experience with high-throughput or semi-automated workflows, process development, assay qualification, data tracking, and reproducible execution in a fast-paced research environment
• High level of attention to detail and commitment to generating high-quality, decision-enabling data
• Experience with cross-functional collaboration in a biotechnology setting, particularly with protein engineering, cell biology, computational biology, translational, or program teams
• Excellent communication skills and ability to present complex scientific concepts, datasets, limitations, and recommendations to colleagues and stakeholders
• Strong track record of managing complex projects, mentoring junior scientists, and driving workstreams from exploratory development through implementation
• Applicants must have ongoing work authorization in the United States that does not require employer sponsorship. Sponsorship will not be provided now or at any time in the future for this position
• Experience with therapeutic genome editing programs and genomic safety packages for candidate nomination, IND-enabling studies, or regulatory-facing documentation
• Experience working with disease-relevant primary cells, ex vivo edited cells, iPSC-derived systems, or other therapeutically relevant model systems
• Familiarity with ML-guided enzyme engineering campaigns and how specificity datasets can inform editor design, model evaluation, and candidate prioritization
• Expertise in protein purification of genome editors for use in biochemical off-target assays

Benefits

• Equity participation
• 401(k) with a strong employer match
• Comprehensive health/dental/vision insurance
• Generous PTO policy and commitment to work-life balance
• Professional development opportunities in a cutting-edge field at the intersection of AI and biology

Company Overview

• Profluent is an AI-first protein design company. It was founded in 2022, and is headquartered in Emeryville, California, USA, with a workforce of 11-50 employees. Its website is https://www.profluent.bio/.